Slivinskiĭ G G, Turganbaeva D A, Naryzhnaia I I
Eksp Onkol. 1984;6(2):42-5.
The sedimentation fractionation of ascitic form of Plyss lymphosarcoma was performed in the linear 1-3% sucrose gradient with 1 g. Fractions were different in cell sizes, protein content and also in intensity of the 3H-thymidine incorporation. Fractions which contained cells in the G1, G1 + S and S + G2 phases were transplanted in vivo in a solid form. Cells with the low sedimentation rate had the maximum growth capacity. The growth rate of G1 + S cells was similar to that of the initial tumour. Cells of high-rate sedimentation were characterized by the low growth activity. The values of D0 for these 3 fractions were 1.64, 2.05 and 2.11 Gy, and those of n 1.9, 2.9 and 1.6, respectively. These differences were not a result of selective injury of cells in fractions or their contamination by nontumour cells.
对腹水型普利斯氏淋巴肉瘤进行沉降分级分离，采用1克线性1 - 3%蔗糖梯度。各组分在细胞大小、蛋白质含量以及3H - 胸腺嘧啶核苷掺入强度方面存在差异。含有处于G1期、G1 + S期和S + G2期细胞的组分以实体形式进行体内移植。沉降速率低的细胞具有最大生长能力。G1 + S期细胞的生长速率与初始肿瘤相似。沉降速率高的细胞具有低生长活性。这三个组分的D0值分别为1.64、2.05和2.11 Gy，n值分别为1.9、2.9和1.6。这些差异并非是组分中细胞的选择性损伤或非肿瘤细胞污染的结果。
