与大鼠肝脏核仁28 S rRNA相关的5.8 S rRNA的12 S前体。

A 12 S precursor to 5.8 S rRNA associated with rat liver nucleolar 28 S rRNA.

作者信息

Dudov K P, Hadjiolova K V, Kermekchiev M B, Stanchev B S, Hadjiolov A A

出版信息

Biochim Biophys Acta. 1983 Jan 20;739(1):79-84. doi: 10.1016/0167-4781(83)90047-7.

Abstract

The pre-rRNA and rRNA components of rat and mouse liver nucleolar RNA were analysed. It was shown that upon denaturation, part of the 32 S pre-rRNA is converted into 28 S rRNA and 12 S RNA. The 12 S RNA from mouse (Mr, 0.36 X 10(6)) is larger than the one from rat (Mr, 0.32 X 10(6). The 12 S RNA chain is intact and resists denaturation treatment. The non-covalent binding of this RNA with nucleolar 28 S rRNA is stronger than that of 5.8 S rRNA with 28 S rRNA. Hybridization with a rat internal-transcribed spacer rDNA fragment identifies 12 S RNA as corresponding to the 5'-end non-conserved segment of 32 S pre-rRNA, including 5.8 S rRNA. The significance of the formation of a 12 S precursor to 5.8 S rRNA in the biogenesis of ribosomes in mammalian cells is discussed.

摘要

对大鼠和小鼠肝脏核仁RNA的前体rRNA和rRNA组分进行了分析。结果表明，变性后，部分32S前体rRNA会转化为28S rRNA和12S RNA。小鼠的12S RNA（Mr，0.36×10⁶）比大鼠的12S RNA（Mr，0.32×10⁶）更大。12S RNA链完整，能抵抗变性处理。该RNA与核仁28S rRNA的非共价结合比5.8S rRNA与28S rRNA的结合更强。与大鼠内部转录间隔区rDNA片段杂交确定12S RNA对应于32S前体rRNA的5'端非保守区段，包括5.8S rRNA。讨论了哺乳动物细胞核糖体生物发生过程中5.8S rRNA的12S前体形成的意义。