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嗜热四膜虫这种低等真核生物前体核糖体RNA加工早期事件中的复杂核酸内切酶切割模式。

Complex endonucleolytic cleavage pattern during early events in the processing of pre-rRNA in the lower eukaryote, Tetrahymena thermophila.

作者信息

Kister K P, Müller B, Eckert W A

出版信息

Nucleic Acids Res. 1983 Jun 11;11(11):3487-502. doi: 10.1093/nar/11.11.3487.

DOI:10.1093/nar/11.11.3487
PMID:6304633
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC325981/
Abstract

We have analysed nuclear RNA from T. thermophila by RNA transfer hybridization using cloned rDNA fragments. A very high number of in vivo intermediates and by-products of rRNA processing were identified. These include putative intermediates of the splicing process and alternative products resulting from temporal variability in various endonucleolytic cleavages. In addition, four small RNA species including only transcribed spacer sequences were detected. These are (1) the IVS RNA (approximately 400 bases), the by-product of the splicing process, (2) a fragment from the internal transcribed spacer (approximately 360 bases), possibly resulting from 3'-end processing of pre-17S rRNA, (3) a fragment comprising most or all of the external transcribed spacer (approximately 600 bases) obviously representing the major by-product of 5'-end processing, and, in addition, (4) a small fragment from the initiation region (approximately 230 bases) which might be a product of premature transcription termination.

摘要

我们利用克隆的rDNA片段，通过RNA转移杂交分析了嗜热栖热菌的核RNA。鉴定出了大量体内rRNA加工的中间体和副产物。这些包括剪接过程的假定中间体以及各种内切核酸酶切割时间变异性产生的替代产物。此外，还检测到了四种仅包含转录间隔序列的小RNA物种。它们分别是：（1）IVS RNA（约400个碱基），剪接过程的副产物；（2）来自内部转录间隔区的一个片段（约360个碱基），可能是前17S rRNA 3'端加工的产物；（3）一个包含大部分或全部外部转录间隔区的片段（约600个碱基），显然是5'端加工的主要副产物；此外，（4）来自起始区域的一个小片段（约230个碱基），可能是过早转录终止的产物。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/6ac7/325981/26b12d40b145/nar00356-0072-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/6ac7/325981/db20bfcada62/nar00356-0069-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/6ac7/325981/26b12d40b145/nar00356-0072-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/6ac7/325981/db20bfcada62/nar00356-0069-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/6ac7/325981/26b12d40b145/nar00356-0072-a.jpg

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Nucleotide sequence of the 5'-terminal coding region for pre-rRNA and mature 17S rRNA in Tetrahymena thermophila rDNA.嗜热四膜虫核糖体DNA中前体核糖体RNA和成熟17S核糖体RNA的5'端编码区核苷酸序列。
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Drosophila DNA topoisomerase I is associated with transcriptionally active regions of the genome.果蝇DNA拓扑异构酶I与基因组的转录活性区域相关联。
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