Rousset B, Tourniaire J, Poncet C, Bornet H
Ann Endocrinol (Paris). 1978;39(1):55-6.
The ability of thyroid tissue from non-toxic goiter to synthesis thyroid hormones was investigated by measuring 131 I-iodide incorporation in T4 in dispersed thyroid cell system. Dispersed thyroid cells were prepared by trypsinization from 5 pathological and 2 Normal thyroid specimens. The patients with non-toxic goiter were euthyroid or in mild hypothyroidism. The capacity of T4 synthesis was decreased in 4 cases and increased in 1 as compared to that of normal thyroid tissue. In one case, the impairement of T4 synthesis seemed to be related to the presence of thyralbumin as the major thyroid iodoprotein. In the 3 other cases with a decreased T4 synthesis, a good correlation was found between the capacity of T4 synthesis in dispersed thyroid cells and the thyroid activity in vivo (6 hr - 132 I-uptake). The discrepancy between in vivo and in vitro data in the case showing an increased T4 synthesis could be explained by the difference in the iodide supply in vitro (5 X 10(-8) M) and in vivo (urinary iodide : 2 microgram/24 hr). Thyroid cell preparations may serve as an experimental model to confirm or invalidate the hypothesis of a defect of thyroid hormone synthesis in non-toxic goiters.
通过在分散的甲状腺细胞系统中测量131I碘化物掺入T4的情况，研究了来自非毒性甲状腺肿的甲状腺组织合成甲状腺激素的能力。从5份病理甲状腺标本和2份正常甲状腺标本中通过胰蛋白酶消化制备分散的甲状腺细胞。患有非毒性甲状腺肿的患者甲状腺功能正常或处于轻度甲状腺功能减退状态。与正常甲状腺组织相比，4例患者的T4合成能力下降，1例增加。在1例中，T4合成受损似乎与作为主要甲状腺碘蛋白的甲状腺白蛋白的存在有关。在其他3例T4合成减少的病例中，发现分散的甲状腺细胞中T4合成能力与体内甲状腺活性（6小时 - 132I摄取）之间存在良好的相关性。在T4合成增加的病例中，体内和体外数据之间的差异可以通过体外（5×10（-8）M）和体内（尿碘：2微克/24小时）碘供应的差异来解释。甲状腺细胞制剂可作为一种实验模型，以证实或否定非毒性甲状腺肿中甲状腺激素合成缺陷的假说。
