无机磷酸盐在马脾铁蛋白中的掺入与释放
Incorporation and release of inorganic phosphate in horse spleen ferritin.
作者信息
Trefry A, Harrison P M
出版信息
Biochem J. 1978 May 1;171(2):313-20. doi: 10.1042/bj1710313.
Abstract
When ferritin is reconstituted from Fe and apoferritin in vitro in the presence of Pi, the product obtained differs both from native ferritin and from ferritin reconstituted in the absence of Pi. When the latter is incubated with Pi the product resembles native ferritin with respect both to the pattern of Pi incorporated per molecule or per Fe atom and to the ease of release of this Pi relative to Fe release. It is concluded that much of the Pi of native ferritin is adsorbed on surfaces of ferritin iron-core crystallites. The results also suggest that Pi is not present at the intracellular site of Fe incorporation into ferritin, but is added after Fe.
摘要
当铁蛋白在体外有磷酸根（Pi）存在的情况下由铁（Fe）和脱铁铁蛋白重构时，所得到的产物既不同于天然铁蛋白，也不同于在没有磷酸根的情况下重构的铁蛋白。当后者与磷酸根一起温育时，就每分子或每个铁原子结合的磷酸根模式以及相对于铁释放而言该磷酸根释放的难易程度而言，产物类似于天然铁蛋白。可以得出结论，天然铁蛋白的大部分磷酸根吸附在铁蛋白铁芯微晶的表面。结果还表明，磷酸根不存在于铁在铁蛋白内的细胞内掺入位点，而是在铁之后添加的。
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