Mayer D E, Rohrer J S, Schoeller D A, Harris D C
Biochemistry. 1983 Feb 15;22(4):876-80. doi: 10.1021/bi00273a026.
The deposition of Fe(II) into ferritin using 18O2 as the oxidant was investigated. Only 3-4% of the oxygen atoms in the FeOOH core of ferritin were derived from the oxidant. This was true whether large (1200-1900 Fe/molecule) or small (220-240 Fe/molecule) amounts of iron were added to apoferritin or when iron was added to ferritin already containing 1000 Fe/molecule. Experiments using H218O in the solvent showed that nearly all of the oxygen atoms in the core were derived from solvent. The stoichiometry of the reaction was close to 2 Fe(II)/O2, instead of the expected value of 4 Fe(II)/O2. Reactions of 18O2 with Fe(II) in the absence of apoferritin had a similar 18O distribution and stoichiometry. Our results are most consistent with the crystal growth model of ferritin iron deposition.
研究了以18O2作为氧化剂将Fe(II)沉积到铁蛋白中的情况。铁蛋白FeOOH核心中只有3-4%的氧原子来自氧化剂。无论向脱铁铁蛋白中添加大量（1200-1900个铁/分子）还是少量（220-240个铁/分子）的铁，或者当铁添加到已经含有1000个铁/分子的铁蛋白中时，都是如此。在溶剂中使用H218O的实验表明，核心中几乎所有的氧原子都来自溶剂。该反应的化学计量接近2 Fe(II)/O2，而不是预期的4 Fe(II)/O2。在没有脱铁铁蛋白的情况下，18O2与Fe(II)的反应具有相似的18O分布和化学计量。我们的结果与铁蛋白铁沉积的晶体生长模型最为一致。
