磷酸酶抑制剂的简易制备及其作用的进一步研究。
Simplified preparation of a phosphatase inhibitor and further studies of its action.
作者信息
Coburn S P, Schaltenbrand W E
出版信息
Biochem J. 1978 May 1;171(2):485-8. doi: 10.1042/bj1710485.
Abstract
1-Pyrrolidinecarbothioic acid (2-pyridylmethylene) hydrazide chelates Zn2+ but not Mg2+. This compound is about twice as effective as EDTA for inhibiting alkaline phosphatase from calf mucosa, and approx. 1000-fold more effective than EDTA for inhibiting acid phosphatase from wheat germ. The compound did not inhibit pyridoxine kinase activity in human leucocytes at the highest concentration tested (33 micron). Therefore it may be a useful tool for either examining or eliminating the effects of phosphatases in complex enzyme systems.
摘要
1-吡咯烷碳硫代酸（2-吡啶基亚甲基）酰肼能螯合锌离子（Zn2+）而非镁离子（Mg2+）。该化合物在抑制小牛黏膜碱性磷酸酶方面的效果约为乙二胺四乙酸（EDTA）的两倍，而在抑制小麦胚芽酸性磷酸酶方面比EDTA有效约1000倍。在测试的最高浓度（33微摩尔）下，该化合物并未抑制人白细胞中的吡哆醇激酶活性。因此，它可能是用于检测或消除复杂酶系统中磷酸酶影响的有用工具。
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