单核糖体、寡核糖体和多核糖体与大鼠肝脏网状膜的结合。
The binding of monoribosomes, oligoribosomes and polyribosomes to reticular membranes from rat liver.
作者信息
Fielder J A, Dani H M, Ridge D, Rabin B R
出版信息
Biochem J. 1978 Apr 15;172(1):109-14. doi: 10.1042/bj1720109.
Abstract
- The activity of the enzyme protein disulphide-isomerase (EC 5.3.4.1) was used to measure the binding of ribosomal fractions to reticular membranes from the liver of unstarved male rats. 2. Membranes degranulated by treatment with KCl plus puromycin and washed by centrifugation through 0.5 M-KCl bound oligoribosomes but not monoribosomes. 3. Substitution of 0.25 M-KCl for 0.5 M-KCl in the wash solution produced membranes that bound monoribosomes and 60 S subunits in addition to oligoribosomes. 4. The binding of all classes of ribosomes to smooth and 'lightly granulated' rough membranes was activated by oestradiol. The hormone activation was much greater with polyribosomes from fed rats than for monoribosomes or oligoribosomes from starved rats. 5. Exposure of rough membranes to centrifugal forces caused them to undergo partial degranulation, as demonstrated by unmasking of protein disulphide-isomerase activity.
摘要
- 利用蛋白质二硫键异构酶（EC 5.3.4.1）的活性来测定核糖体组分与未饥饿雄性大鼠肝脏网状膜的结合情况。2. 用氯化钾加嘌呤霉素处理后脱颗粒并通过0.5M氯化钾离心洗涤的膜结合寡核糖体，但不结合单核糖体。3. 在洗涤液中用0.25M氯化钾替代0.5M氯化钾，产生的膜除了结合寡核糖体外，还结合单核糖体和60S亚基。4. 雌二醇可激活所有类型核糖体与光滑和“轻度颗粒化”粗面膜的结合。与饥饿大鼠的单核糖体或寡核糖体相比，喂食大鼠的多核糖体的激素激活作用要强得多。5. 粗面膜暴露于离心力下会使其发生部分脱颗粒，这可通过蛋白质二硫键异构酶活性的暴露来证明。
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本文引用的文献
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Protein measurement with the Folin phenol reagent.使用福林酚试剂进行蛋白质测定。
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A simple method for the isolation and purification of total lipides from animal tissues.一种从动物组织中分离和纯化总脂质的简单方法。
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Studies on the reattachment of ribosomes and ribosomal subunits to ribosome-free membranes prepared from rat liver microsomes by means of lithium chloride.关于通过氯化锂将核糖体及核糖体亚基重新附着到从大鼠肝脏微粒体制备的无核糖体膜上的研究。
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The isolation of polysomes from rat liver. Contamination by membrane proteins with high affinity to ribosomes.从大鼠肝脏中分离多核糖体。受与核糖体具有高亲和力的膜蛋白污染。
Biochim Biophys Acta. 1971 Apr 8;232(4):705-16.
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Role for steroid hormones in the interaction of ribosomes with the endoplasmic membranes of rat liver.类固醇激素在大鼠肝脏核糖体与内质网膜相互作用中的作用。
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