用于糙面内质网体外重建的大鼠肝脏细胞组分中的肽基-tRNA水解酶和核糖核酸酶活性
Peptidyl - tRNA hydrolase and RNase activities in cell fractions of rat liver used in in vitro reconstitution of rough membrane.
作者信息
Hochberg A A, Czosnek H H, Ziv E
出版信息
Nucleic Acids Res. 1975 Jun;2(6):943-50. doi: 10.1093/nar/2.6.943.
Abstract
Peptidyl-tRNA hydrolase and RNase activities have been studied in those fractions of rat liver, which are used in in vitro reconstitution of rough membrane, because these enzymes may interfere with the in vitro reconstitution. It was found that smooth membrane has an active peptidyl-tRNA hydrolase, while the other fractions tested, polyribosomes, rough membrane, stripped rough membrane and the post-microsomal supernatant had no, or very low, peptidyl-tRNA hydrolase activity. Polyribosomes, rough and stripped rough membrane have RNase activity; this activity could be completely inhibited by rat liver RNase inhibitor. It is shown that RNase inhibitor is an obligatory component in in vitro experiments, in which rough membrane is reconstituted from stripped rough membrane, ribosomes and mRNA.
摘要
已对大鼠肝脏中用于粗面内质网体外重建的那些组分中的肽基 - tRNA水解酶和核糖核酸酶活性进行了研究，因为这些酶可能会干扰体外重建。结果发现，滑面内质网具有活性肽基 - tRNA水解酶，而测试的其他组分，即多核糖体、粗面内质网、脱膜粗面内质网和微粒体后上清液，要么没有肽基 - tRNA水解酶活性，要么活性非常低。多核糖体、粗面和脱膜粗面内质网具有核糖核酸酶活性；这种活性可被大鼠肝脏核糖核酸酶抑制剂完全抑制。结果表明，在从脱膜粗面内质网、核糖体和mRNA体外重建粗面内质网的实验中，核糖核酸酶抑制剂是必不可少的成分。
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本文引用的文献
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A small particulate component of the cytoplasm.细胞质的一种小颗粒成分。
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The effects of aflatoxin B(1) and steroid hormones on polysome binding to microsomal membranes as measured by the activity of an enzyme catalysing disulphide interchange.通过催化二硫键交换的酶活性来测定黄曲霉毒素B（1）和类固醇激素对多核糖体与微粒体膜结合的影响。
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The binding of polysomes to smooth membranes of rat liver promoted by steroid hormones and extracts from either rough endoplasmic reticulum or from polysomes of the opposite sex.甾体激素以及来自糙面内质网或异性多核糖体的提取物可促进大鼠肝脏多核糖体与光滑膜的结合。
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Isolation and properties of polyribosomes and fragments of the endoplasmic reticulum from rat liver.大鼠肝脏多核糖体和内质网片段的分离及特性
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Active hybrid 80 s particles formed from subunits of rat, rabbit and protozoan (Tetrahymena pyriformis) ribosomes.由大鼠、兔子和原生动物（梨形四膜虫）核糖体亚基形成的活性杂交80s颗粒。
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