吸附的可溶性[3H]弹性蛋白作为蛋白酶活性的底物。一种新的微量测定技术。

Adsorbed soluble [3H]elastin as substrate for proteinase activity. A new microassay technique.

作者信息

Jensen H S

出版信息

Biochem J. 1984 Mar 1;218(2):645-8. doi: 10.1042/bj2180645.

DOI:10.1042/bj2180645

原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC1153386/

Abstract

A microassay for elastase activity has been designed. Microtubes are coated with soluble elastin substrate by passive adsorption to the tube walls. On contact with enzymes in test samples, radioactivity is released into the sample buffer, which is then transferred to vials containing scintillation fluid without further processing. Porcine pancreatic elastase (EC 3.4.21.11) is easily detected in concentrations down to 8 micrograms/litre. The interassay coefficient of variation (CV) was below 10% for values above 50 micrograms/litre. The assay is rapid, precise, economical and sensitive.

摘要

已设计出一种用于弹性蛋白酶活性的微量测定法。通过被动吸附至管壁，将微量管涂覆有可溶性弹性蛋白底物。当与测试样品中的酶接触时，放射性物质释放到样品缓冲液中，然后将其转移到含有闪烁液的小瓶中，无需进一步处理。猪胰弹性蛋白酶（EC 3.4.21.11）在低至8微克/升的浓度下即可轻松检测到。对于高于50微克/升的值，测定间变异系数（CV）低于10%。该测定法快速、精确、经济且灵敏。

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