四种弹性蛋白酶活性测量方法的评估。

An evaluation of four methods for the measurement of elastase activity.

作者信息

McGillivray D H, Burnett D, Afford S C, Stockley R A

出版信息

Clin Chim Acta. 1981 Apr 9;111(2-3):289-94. doi: 10.1016/0009-8981(81)90200-x.

Abstract

The precision of four different methods for the measurement of low levels of porcine pancreatic elastase was assessed using a synthetic substrate and by three other methods using the natural substrate elastin. The most precise method used the synthetic substrate Succ(Ala)3NA (within-batch coefficient of variation = 1.9%, between-batch coefficient of variation = 2.5%). Fluorescein-labelled elastin was the most precise of those methods using elastin as substrate (within-batch coefficient of variation = 5.1%, between-batch coefficient of variation = 5.5%).

摘要

使用合成底物以及其他三种使用天然底物弹性蛋白的方法，评估了四种不同方法测量低水平猪胰弹性蛋白酶的精密度。最精确的方法使用合成底物琥珀酰（丙氨酸）3萘胺（批内变异系数=1.9%，批间变异系数=2.5%）。荧光素标记的弹性蛋白是那些以弹性蛋白为底物的方法中最精确的（批内变异系数=5.1%，批间变异系数=5.5%）。