Ferro M, Nanni G
Boll Ist Sieroter Milan. 1978 Mar 31;57(1):60-4.
Pronase digested sheep red blood cell stromata were employed as probe in order to investigate erythrocyte membrane arrangement by immunological way. Antiserum from rabbits immunized with erythrocyte ghost pronase residue assayed for its hemolytic activity against intact sheep red cells, showed an high titer, in a good agreement with the presence in the same residue of externally located membrane antigens. Immunoelectrophoretic analysis was performed between the solubilized residue and precipitins produced by rabbits immunized respectively with the following antigens: intact sheep red blood cells (SRBC), sheep erythrocyte stromata (SRBCS), lipid complex (LC), sphingomyelin complex (SF), stromata after phospholipase A treatment (SPLA), stromata after phospholipase C treatment (SPLC), stromata after phospholipases A and C treatment (SPLAC), and pronase treated stromata (SP). Antigen/antibody reaction with anti-SP antiserum showed an additional precipitation line: this fact is discussed in view of a possible enrichment of the fraction after pronase stromata digestion, and/or enhancement of the immunogenicity.
为了通过免疫学方法研究红细胞膜的排列，使用了链霉蛋白酶消化的绵羊红细胞基质作为探针。用红细胞血影链霉蛋白酶残渣免疫的兔抗血清针对完整绵羊红细胞的溶血活性进行检测，结果显示其效价很高，这与同一残渣中位于外部的膜抗原的存在情况高度一致。对溶解的残渣与分别用以下抗原免疫的兔产生的沉淀素进行了免疫电泳分析：完整绵羊红细胞（SRBC）、绵羊红细胞基质（SRBCS）、脂质复合物（LC）、鞘磷脂复合物（SF）、磷脂酶A处理后的基质（SPLA）、磷脂酶C处理后的基质（SPLC）、磷脂酶A和C处理后的基质（SPLAC）以及链霉蛋白酶处理后的基质（SP）。抗原/抗体与抗SP抗血清的反应显示出一条额外的沉淀线：鉴于链霉蛋白酶处理基质消化后该组分可能的富集和/或免疫原性的增强，对这一事实进行了讨论。
