Marinari U M, Averame M M, Casu A, Nanni G
Ital J Biochem. 1977 May-Jun;26(3):232-44.
The composition of single carbohydrate classes of intact and pronase treated sheep erythrocyte membranes has been studied. In comparison with the data obtained from untreated stromata after proteolytic digestion the amount of each class of sugars is decreased. A high disappearance of sialic acids and hexosamines can be observed. Nevertheless if the total sugar content is referred to the residual protein content (16% of the native proteins, an enrichment in carbohydrates, specially neutral hexoses, can be observed. The results indicate that most of the carbohydrate fractions solubilized by pronase treatment are sialic acids and hexosamines. Different molar ratios obtained for the single carbohydrate classes in comparison with NANA before and after pronase treatment suggest a microheterogeneity of the glycoprotein structure of sheep erythrocyte membranes. By the use of several analytical methods the residue obtained after pronase treatment shows two major fractions containing proteins, lipids and carbohydrates. The data are discussed in view of the possible arrangement of lipo-glycoproteins in sheep erythrocyte membrane.
已对完整的和经链霉蛋白酶处理的绵羊红细胞膜中单一碳水化合物类别的组成进行了研究。与蛋白水解消化后未处理的基质所获得的数据相比，每类糖的量均有所减少。可以观察到唾液酸和己糖胺大量消失。然而，如果将总糖含量与残留蛋白质含量（天然蛋白质的16%）相关联，则可以观察到碳水化合物的富集，特别是中性己糖。结果表明，经链霉蛋白酶处理后溶解的大部分碳水化合物部分是唾液酸和己糖胺。与链霉蛋白酶处理前后的NANA相比，单一碳水化合物类别的不同摩尔比表明绵羊红细胞膜糖蛋白结构存在微不均一性。通过使用几种分析方法，链霉蛋白酶处理后获得的残留物显示出两个主要部分，包含蛋白质、脂质和碳水化合物。鉴于脂糖蛋白在绵羊红细胞膜中的可能排列，对这些数据进行了讨论。
