早产新生儿中硫酸化不足的尿糖胺聚糖

Undersulfated urinary glycosaminoglycans in preterm newborns.

作者信息

Coppa G V, Gabrielli O, Maiorana A, Sani S, Benincasa A, Belsito M, Montali U

出版信息

Biol Neonate. 1978;33(1-2):18-24. doi: 10.1159/000241046.

Abstract

The glycosaminoglycans (GAG) isolated from pooled urine of preterm newborns were fractionated by stepwise elution from a Dowex 1-X2 column. Analytical reactions, cellulose acetate electrophoresis and enzymatic digestions with chondroitinases and testicular hyaluronidase were performed on each fraction. Chondroitin-4-sulfate and chondroitin-6-sulfate constitute about 60% of urinary GAG; heparan sulfate amounts to about 20%, while chondroitin represents 12% of total GAG; dermatan sulfate, hyaluronic acid and keratan sulfate are present in small traces. Approximately 30% of the total is constituted by nonsulfated GAG.

摘要

从早产新生儿混合尿液中分离出的糖胺聚糖（GAG），通过从Dowex 1-X2柱上进行分步洗脱进行分级分离。对每个级分进行分析反应、醋酸纤维素电泳以及用软骨素酶和睾丸透明质酸酶进行酶消化。硫酸软骨素-4和硫酸软骨素-6约占尿GAG的60%；硫酸乙酰肝素约占20%，而软骨素占总GAG的12%；硫酸皮肤素、透明质酸和硫酸角质素含量极少。总GAG中约30%由非硫酸化GAG构成。