Nikitina Z K, Shishkin S S, Khoroshkov Iu A, Debov S S
Biull Eksp Biol Med. 1978 May;85(5):533-7.
The separation in a sucrose gradient of the "myofibrillar" fraction of normal and autolysed muscle tissue gave 4 components. During the post-mortem destruction of the tissue there was observed a slight decrease of the "myofibrillar" fraction yield and also certain changes in the distribution of protein between different components. Under the selected conditions the RNA-ase activity was found in all 4 components. During the course of autolysis the enzymatic activity increased in the whole "myofibrillar" fraction, as well as in the lysosomal-mitochondrial components of "myofibrillae".
正常和自溶肌肉组织的“肌原纤维”部分在蔗糖梯度中的分离产生了4种成分。在组织的死后破坏过程中,观察到“肌原纤维”部分的产量略有下降,并且不同成分之间的蛋白质分布也有一定变化。在选定条件下,在所有4种成分中均发现了RNA酶活性。在自溶过程中,整个“肌原纤维”部分以及“肌原纤维”的溶酶体-线粒体成分中的酶活性均增加。