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[正常和自溶肌肉组织“肌原纤维”部分的核糖核酸酶活性]

[Ribonuclease activity of the "myofibrillar" fraction of normal and autolyzed muscle tissue].

作者信息

Nikitina Z K, Shishkin S S, Khoroshkov Iu A, Debov S S

出版信息

Biull Eksp Biol Med. 1978 May;85(5):533-7.

PMID:656599
Abstract

The separation in a sucrose gradient of the "myofibrillar" fraction of normal and autolysed muscle tissue gave 4 components. During the post-mortem destruction of the tissue there was observed a slight decrease of the "myofibrillar" fraction yield and also certain changes in the distribution of protein between different components. Under the selected conditions the RNA-ase activity was found in all 4 components. During the course of autolysis the enzymatic activity increased in the whole "myofibrillar" fraction, as well as in the lysosomal-mitochondrial components of "myofibrillae".

摘要

正常和自溶肌肉组织的“肌原纤维”部分在蔗糖梯度中的分离产生了4种成分。在组织的死后破坏过程中,观察到“肌原纤维”部分的产量略有下降,并且不同成分之间的蛋白质分布也有一定变化。在选定条件下,在所有4种成分中均发现了RNA酶活性。在自溶过程中,整个“肌原纤维”部分以及“肌原纤维”的溶酶体-线粒体成分中的酶活性均增加。

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