Nikitina Z K, Shishkin S S, Tomashevich S V, Debov S S
Vopr Med Khim. 1978 Mar-Apr;24(2):262-70.
Comparative histochemical study of RNA content in normal and autolyzed within 72 hrs muscle tissue showed that the nucleic acid well retained during autolysis but the tissue was simultaneously destructed. Polymerization of rRNA in autolyzed tissue proved to depend on temperature of dead animals storage. An increase of temperature from 0 degrees to 21 degrees did not affect the rRNA yield. But amount of latent breaks, which were found in rRNA by methods of formamide and temperature treatment, was increased depending on increase in temperature and on length of animal storage after death. The latent breaks appear to be accumulated in animal tissues during autolysis.
对正常及在72小时内自溶的肌肉组织中RNA含量进行的比较组织化学研究表明,核酸在自溶过程中保存良好,但组织同时遭到破坏。自溶组织中rRNA的聚合作用被证明取决于死亡动物的储存温度。温度从0摄氏度升至21摄氏度并不影响rRNA的产量。但是,通过甲酰胺和温度处理方法在rRNA中发现的潜在断裂数量,会随着温度升高和动物死后储存时间的延长而增加。潜在断裂似乎在自溶过程中在动物组织中积累。