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[大鼠骨骼肌自溶过程中核糖体RNA的降解]

[Ribosomal RNA degradation in rat skeletal muscle in the process of autolysis].

作者信息

Nikitina Z K, Shishkin S S, Tomashevich S V, Debov S S

出版信息

Vopr Med Khim. 1978 Mar-Apr;24(2):262-70.

PMID:664453
Abstract

Comparative histochemical study of RNA content in normal and autolyzed within 72 hrs muscle tissue showed that the nucleic acid well retained during autolysis but the tissue was simultaneously destructed. Polymerization of rRNA in autolyzed tissue proved to depend on temperature of dead animals storage. An increase of temperature from 0 degrees to 21 degrees did not affect the rRNA yield. But amount of latent breaks, which were found in rRNA by methods of formamide and temperature treatment, was increased depending on increase in temperature and on length of animal storage after death. The latent breaks appear to be accumulated in animal tissues during autolysis.

摘要

对正常及在72小时内自溶的肌肉组织中RNA含量进行的比较组织化学研究表明,核酸在自溶过程中保存良好,但组织同时遭到破坏。自溶组织中rRNA的聚合作用被证明取决于死亡动物的储存温度。温度从0摄氏度升至21摄氏度并不影响rRNA的产量。但是,通过甲酰胺和温度处理方法在rRNA中发现的潜在断裂数量,会随着温度升高和动物死后储存时间的延长而增加。潜在断裂似乎在自溶过程中在动物组织中积累。

相似文献

1
[Ribosomal RNA degradation in rat skeletal muscle in the process of autolysis].[大鼠骨骼肌自溶过程中核糖体RNA的降解]
Vopr Med Khim. 1978 Mar-Apr;24(2):262-70.
2
[In vitro degradation of autolyzed and nonautolyzed RNP-particles from rat skeletal muscles].[大鼠骨骼肌自溶和未自溶核糖核蛋白颗粒的体外降解]
Biokhimiia. 1977 Jun;42(6):1045-51.
3
[Structural and functional changes in myosin from rat skeletal muscles during postmortem autolysis].
Vopr Med Khim. 1988 Nov-Dec;34(6):76-81.
4
[Postmortem decomposition of skeletal muscle RNA].[骨骼肌RNA的死后分解]
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[Effect of postmortem autolysis on the 40S ribosomal subunit proteins of rat skeletal muscles].[死后自溶对大鼠骨骼肌40S核糖体亚基蛋白的影响]
Vopr Med Khim. 1980 Jul-Aug;26(4):476-80.
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[Postmortem inhibition of RNA synthesis in the liver and skeletal muscles of rats].[大鼠肝脏和骨骼肌中RNA合成的死后抑制]
Vopr Med Khim. 1979 Jul-Aug;25(4):408-14.
7
[Changes in human skeletal muscle proteins during autolysis studied by two-dimensional electrophoresis].
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8
Comparison of postmortem autolysis in cardiac and skeletal muscle.心肌与骨骼肌死后自溶的比较。
J Forensic Sci. 1993 Sep;38(5):1187-93.
9
[Activation of latent RNAse activity of RNP particles from rat skeletal muscles].[大鼠骨骼肌核糖核蛋白颗粒潜在核糖核酸酶活性的激活]
Biokhimiia. 1977 Apr;42(4):712-9.
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