Shishkin S S, Nikitina Z K
Vopr Med Khim. 1977 May-Jun(3):346-51.
RNAs, isolated from rat sceletal muscles by heat and cold phenol treatment within various periods after animal death, were analyzed by means of centrifugation in sucrose gradient. Content of 28 S RNA was found to be decreased by 7-8% within 3 days of autolysis with maintaining of the total ribosomal RNA yield. These data suggest that nucleases were present in microsomal and crude myofibrillar fractions and their activity was increased duing autolysis.
通过在动物死亡后的不同时间段内采用热酚法和冷酚法从大鼠骨骼肌中分离出RNA，并通过蔗糖梯度离心法进行分析。结果发现，在自溶3天内，28S RNA的含量下降了7 - 8%，而核糖体RNA的总产量保持不变。这些数据表明，核酸酶存在于微粒体和粗肌原纤维组分中，并且它们的活性在自溶过程中增强。
