Harbitz I, Wallin B, Hauge J G, Mørland J
Biochem Pharmacol. 1984 Nov 1;33(21):3465-70. doi: 10.1016/0006-2952(84)90121-7.
Rat liver parenchymal cells were incubated in the presence and absence of ethanol (80 mM). Polysomes were isolated and analysed on sucrose gradients. Ethanol was shown to inhibit the incorporation of 14C-valine into proteins, result in a shift in the distribution of polysomes towards smaller sizes, inhibit the formation of 40S initiation complexes, and diminish the concentration of glucose-6-phosphate in the hepatocytes. Addition of 4-methylpyrazole (0.5 mM) partially prevented the inhibition of protein synthesis and completely restored the polysomal distribution. It is concluded that ethanol inhibits protein synthesis partly by a mechanism linked to ethanol metabolism. This effect takes place at the level of initiation and may be mediated by a reduced gluconeogenesis.
将大鼠肝实质细胞在有和没有乙醇（80 mM）的情况下进行孵育。分离多核糖体并在蔗糖梯度上进行分析。结果显示，乙醇可抑制14C-缬氨酸掺入蛋白质，导致多核糖体分布向较小尺寸偏移，抑制40S起始复合物的形成，并降低肝细胞中葡萄糖-6-磷酸的浓度。添加4-甲基吡唑（0.5 mM）可部分阻止蛋白质合成的抑制，并完全恢复多核糖体分布。得出的结论是，乙醇部分通过与乙醇代谢相关的机制抑制蛋白质合成。这种作用发生在起始水平，可能由糖异生减少介导。
