专性化能自养硫杆菌的遗传转化
Genetic transformation of obligately chemolithotrophic thiobacilli.
作者信息
Yankofsky S A, Gurevich R, Grimland N, Stark A A
出版信息
J Bacteriol. 1983 Feb;153(2):652-7. doi: 10.1128/jb.153.2.652-657.1983.
Abstract
Genetic transformation of Thiobacaillus thioparus auxotrophs to prototrophy was obtained at frequencies of up to 10(-2) when proliferating cell populations were exposed to chromosomal DNA from a nutritionally independent strain of the same bacterium. The rate at which transformation occurred depended on recipient growth rate and could be drastically reduced by depriving otherwise competent cells of either nitrogen or exogenous energy substrate. Interspecies marker transfer was also shown among several obligately chemolithotrophic members of the genus.
摘要
当增殖细胞群体暴露于来自同一细菌的营养自主菌株的染色体DNA时，嗜硫硫杆菌营养缺陷型向原养型的遗传转化频率高达10^(-2)。转化发生的速率取决于受体的生长速率，并且通过剥夺原本有转化能力的细胞的氮源或外源能量底物，转化速率可能会大幅降低。在该属的几个专性化能自养成员之间也显示出种间标记转移。
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