Stark A A, Yankofsky S A
J Bacteriol. 1981 Dec;148(3):966-72. doi: 10.1128/jb.148.3.966-972.1981.
Amino acid transport in amino acid auxotrophs of Thiobacillus thioparus was enhanced during growth on rate-limiting amino acid concentration. A pleiotropic mutation enhanced general amino acid transport as manifested by higher values of Vmax of amino acid transport. Affinity constants remained unaltered. Mutants with enhanced transport properties did not show changes in oxidation of thiosulfate, did not oxidize various organic compounds, and did not increase the heterotrophic potential of T. thioparus. The mutations for enhanced transport caused increased synthesis of amino acid transport system components. A method for genetic transformation of T. thioparus is described.
在以限速氨基酸浓度生长期间，硫杆菌属嗜硫菌的氨基酸营养缺陷型中的氨基酸转运得到增强。一个多效性突变增强了一般氨基酸转运，这表现为氨基酸转运的Vmax值更高。亲和常数保持不变。具有增强转运特性的突变体在硫代硫酸盐氧化方面没有变化，不氧化各种有机化合物，也没有增加嗜硫菌的异养潜力。增强转运的突变导致氨基酸转运系统组分的合成增加。描述了一种嗜硫菌的遗传转化方法。
