Ulmer K M
Science. 1983 Feb 11;219(4585):666-71. doi: 10.1126/science.6572017.
The prospects for protein engineering, including the roles of x-ray crystallography, chemical synthesis of DNA, and computer modelling of protein structure and folding, are discussed. It is now possible to attempt to modify many different properties of proteins by combining information on crystal structure and protein chemistry with artificial gene synthesis. Such techniques offer the potential for altering protein structure and function in ways not possible by any other method.
本文讨论了蛋白质工程的前景，包括X射线晶体学、DNA化学合成以及蛋白质结构与折叠的计算机建模所起的作用。现在，通过将晶体结构和蛋白质化学信息与人工基因合成相结合，有可能尝试改变蛋白质的许多不同特性。这些技术提供了以其他任何方法都无法实现的方式改变蛋白质结构和功能的潜力。
