Inazu N, Kogo H, Aizawa Y
Biochim Biophys Acta. 1983 Jan 7;750(1):98-104. doi: 10.1016/0005-2760(83)90208-4.
Effects of pregnant mare serum gonadotropin and human chorionic gonadotropin on the formation of 13,14-dihydroprostaglandin F2 alpha, a biologically active compound, were investigated in rat ovarian homogenate. The mass number of the compound, which was formed prostaglandin F2 alpha via 13,14-dihydro-15-ketoprostaglandin F2 alpha in rat ovarian homogenate but was not produced in rat homogenate, accorded with that of the authentic 13,14-dihydroprostaglandin F2 alpha by negative ion chemical ionization mass spectrometry. In the present experiment, the radioactivity of [3H]prostaglandin F2 alpha added to ovarian homogenate was decreased linearly and immediately until the incubation time of 10 min. The formation of 13,14-dihydroprostaglandin F2 alpha was increased up to 60 min. The formation of 13,14-dihydroprostaglandin F2 alpha from prostaglandin F2 alpha was markedly increased by pregnant mare serum gonadotropin and human chorionic gonadotropin. However, there was no additive or synergistic effect of these hormones. The formation of 13,14-dihydroprostaglandin F2 alpha from 13,14-dihydro-15-ketoprostaglandin F2 alpha weas also greatly stimulated by pregnant mare serum gonadotropin and human chorionic gonadotropin. The formation of 13,14-dihydro-15-ketoprostaglandin F2 alpha steeply declined until 24 h after treatment with human chorionic gonadotropin in pregnant mare serum gonadotropin-primed rats. In contrast, the formation of 13,14-dihydroprostaglandin F2 alpha was markedly increased until 24 h after human chorionic gonadotropin treatment, and the level was about 2.5-fold higher than that at 0 h, 48 h after injection of pregnant mare serum gonadotropin.
在大鼠卵巢匀浆中研究了孕马血清促性腺激素和人绒毛膜促性腺激素对生物活性化合物13,14 - 二氢前列腺素F2α形成的影响。该化合物在大鼠卵巢匀浆中通过13,14 - 二氢 - 15 - 酮前列腺素F2α形成前列腺素F2α，但在大鼠匀浆中不产生，其质量数通过负离子化学电离质谱法与 authentic 13,14 - 二氢前列腺素F2α的质量数一致。在本实验中，添加到卵巢匀浆中的[3H]前列腺素F2α的放射性在孵育时间达到10分钟之前立即呈线性下降。13,14 - 二氢前列腺素F2α的形成在60分钟内增加。孕马血清促性腺激素和人绒毛膜促性腺激素显著增加了由前列腺素F2α形成的13,14 - 二氢前列腺素F2α。然而，这些激素没有相加或协同作用。孕马血清促性腺激素和人绒毛膜促性腺激素也极大地刺激了由13,14 - 二氢 - 15 - 酮前列腺素F2α形成的13,14 - 二氢前列腺素F2α。在孕马血清促性腺激素预处理的大鼠中，用人绒毛膜促性腺激素处理后，13,14 - 二氢 - 15 - 酮前列腺素F2α的形成在24小时内急剧下降。相比之下，用人绒毛膜促性腺激素处理后，13,14 - 二氢前列腺素F2α的形成在24小时内显著增加，且该水平比注射孕马血清促性腺激素后0小时时高约2.5倍，在48小时时亦是如此。
