McCulloch J, Kelly P A, Uddman R, Edvinsson L
Proc Natl Acad Sci U S A. 1983 Mar;80(5):1472-6. doi: 10.1073/pnas.80.5.1472.
The quantitative autoradiographic 2-deoxy-[14C]glucose technique has been used with conscious rats to investigate the functional consequences (reflected as alterations in local rates of glucose utilization) of unilateral intrastriatal administration of vasoactive intestinal polypeptide. Intrastriatal administration of vasoactive intestinal polypeptide (20 pmol) significantly increased local glucose utilization in the injected striatum, where the increased use was localized in small punctate areas (100-500 microns wide in coronal sections) scattered throughout the nucleus at considerable distances (up to 4 mm) from the injection site. Significantly increased glucose utilization after intrastriatal injection of vasoactive intestinal polypeptide was observed in a number of regions (e.g., substantia nigra pars compacta, entopeduncular nucleus, lateral habenular nucleus, entorhinal, pyriform cortices, and amygdala) with known primary or secondary neuronal connections with the caudate nucleus. These alterations in glucose utilization were highly focal in nature, with the majority (40 of the 50 examined) of brain regions displaying unaltered rates of glucose utilization. The data provide evidence, obtained in conscious animals, that vasoactive intestinal polypeptide can modify functional processes in the caudate nucleus.
采用定量放射自显影2-脱氧-[14C]葡萄糖技术，对清醒大鼠进行研究，以探讨纹状体内单侧注射血管活性肠肽的功能后果（以局部葡萄糖利用率的改变来反映）。纹状体内注射血管活性肠肽（20皮摩尔）可显著增加注射侧纹状体的局部葡萄糖利用率，增加的利用集中在小的点状区域（冠状切片中宽100 - 500微米），这些区域分散在整个核内，距注射部位相当远（达4毫米）。在一些与尾状核有已知一级或二级神经元联系的区域（如黑质致密部、内苍白球核、外侧缰核、内嗅皮质、梨状皮质和杏仁核），观察到纹状体内注射血管活性肠肽后葡萄糖利用率显著增加。这些葡萄糖利用率的改变本质上高度局限，大多数（50个检查区域中的40个）脑区的葡萄糖利用率未改变。这些数据提供了在清醒动物身上获得的证据，表明血管活性肠肽可改变尾状核的功能过程。
