Kasugai S
Bull Tokyo Med Dent Univ. 1983 Mar;30(1):9-15.
An autoradiographic study using 3H-thymidine (3H-TdR) was carried out to investigate which cells migrate from the pulp explant of the rat incisor in vitro. The cells close to the arterioles (perivascular cells) were especially labeled after 3H-TdR incubation following the dissection of the pulp explant. In tracing these 3H-TdR-labeled perivascular cells, about 15 per cent of the outgrowth cells were the perivascular cells after 2 days of culture and these perivascular cells had a high mitotic activity in vitro. On the other hand, it was ascertained that the odontoblasts in the pulp explant underwent necrotic changes and that the odontoblasts had no ability to migrate from the pulp explant.
采用³H-胸腺嘧啶核苷（³H-TdR）进行了一项放射自显影研究，以探究在体外培养条件下，大鼠切牙髓外植体中哪些细胞会发生迁移。牙髓外植体解剖后，经³H-TdR孵育，靠近小动脉的细胞（血管周围细胞）被特别标记。在追踪这些³H-TdR标记的血管周围细胞时发现，培养2天后，约15%的生长细胞为血管周围细胞，且这些血管周围细胞在体外具有较高的有丝分裂活性。另一方面，已确定牙髓外植体中的成牙本质细胞发生了坏死性变化，且成牙本质细胞无能力从牙髓外植体迁移。
