Upreti G C, deAntueno R J, Wood R
J Natl Cancer Inst. 1983 Mar;70(3):559-66.
Enriched subcellular fractions of nuclei, mitochondria, endoplasmic reticulum (ER), plasma membrane, and cytosol were prepared from liver and hepatoma 7288CTC taken from male inbred BUF rats. Purity was established by marker enzyme activities and distribution of DNA, RNA, sialic acids, total phospholipids, and cholesterol. The subcellular fractions of hepatoma differed from those of liver: 5'-Nucleotidase activity was elevated in ER and mitochondria, cytosol RNA was increased, and cholesterol was elevated in all hepatoma subcellular fractions. Neutral lipid classes of hepatoma subcellular fractions differed quantitatively from those of liver: Hepatoma nuclei and mitochondria contained elevated levels of free fatty acids (FFA) and triglycerides (TG). Generally, the fatty acid profiles of FFA, TG, and sterol esters from hepatoma subcellular fractions were more uniform and showed less organelle specificity than did liver. Hepatoma FFA and TG contained lower percentages of palmitate and higher percentages of stearate in all subcellular fractions than did liver. The sterol esters from most hepatoma subcellular fractions compared to those from liver were characterized by much higher levels of long-chain fatty acids of 20 carbon atoms or longer. The oleate-to-vaccenate ratio in FFA of liver subcellular fractions exhibited some specificity, but not that of hepatoma subcellular fractions. The oleate-to-vaccenate ratio in the acyl chains of liver and hepatoma TG did not reveal organelle specificity.
从雄性近交BUF大鼠的肝脏和肝癌7288CTC中制备了富含细胞核、线粒体、内质网（ER）、质膜和胞质溶胶的亚细胞级分。通过标记酶活性以及DNA、RNA、唾液酸、总磷脂和胆固醇的分布确定纯度。肝癌的亚细胞级分与肝脏的不同：内质网和线粒体中的5'-核苷酸酶活性升高，胞质溶胶RNA增加，并且所有肝癌亚细胞级分中的胆固醇升高。肝癌亚细胞级分的中性脂质类别在数量上与肝脏的不同：肝癌细胞核和线粒体中游离脂肪酸（FFA）和甘油三酯（TG）的水平升高。一般来说，肝癌亚细胞级分中FFA、TG和甾醇酯的脂肪酸谱比肝脏更均匀，且细胞器特异性更低。与肝脏相比，肝癌所有亚细胞级分中的FFA和TG含有的棕榈酸百分比更低，硬脂酸百分比更高。与肝脏相比，大多数肝癌亚细胞级分中的甾醇酯的特征是含有更高水平的20个碳原子或更长的长链脂肪酸。肝脏亚细胞级分的FFA中的油酸与牛痘酸之比表现出一定的特异性，但肝癌亚细胞级分没有。肝脏和肝癌TG的酰基链中的油酸与牛痘酸之比未显示细胞器特异性。
