Boldt D H, Zimmer S G
J Natl Cancer Inst. 1983 May;70(5):943-8.
Transformation of rat embryo fibroblasts by human adenovirus type 2 (Ad2) results in the production of a series of cell lines that cover a spectrum of malignancy from nontumorigenic to highly tumorigenic in a single species. A panel of plant lectins was used to study surface characteristics of these cell lines that might correlate with tumorigenicity. Because of the complex nature of lectin-cell surface interactions, a number of parameters were determined; they included numbers and densities of lectin receptors, binding affinities, and receptor mobilities. The lectins from Lens culinaris, Lotus tetragonolobus, and Ricinus communis were found to be the most useful for differentiating among the various Ad2-transformed cell lines. In general, the more tumorigenic cell lines were characterized by high numbers of lectin receptors, high percentages of lectin-binding cells, and heterogeneous distributions of receptors from cell to cell. In contrast, the nontumorigenic and the weakly tumorigenic cell lines were characterized by low numbers of lectin receptors present on a minority of cells within each population and a more homogeneous distribution of these receptors from cell to cell. These data demonstrate that lectins can identify surface properties that appear to correlate with malignant potential in the Ad2-transformed cell lines.
人2型腺病毒（Ad2）对大鼠胚胎成纤维细胞的转化导致产生了一系列细胞系，这些细胞系涵盖了单一物种中从非致瘤性到高致瘤性的恶性程度范围。使用一组植物凝集素来研究这些细胞系可能与致瘤性相关的表面特征。由于凝集素与细胞表面相互作用的性质复杂，测定了许多参数；包括凝集素受体的数量和密度、结合亲和力以及受体迁移率。发现来自小扁豆、四角豆和蓖麻的凝集素对于区分各种Ad2转化的细胞系最有用。一般来说，致瘤性更强的细胞系的特征是凝集素受体数量多、凝集素结合细胞百分比高以及细胞间受体分布不均一。相比之下，非致瘤性和弱致瘤性细胞系的特征是每个群体中少数细胞上存在的凝集素受体数量少，且这些受体在细胞间的分布更均匀。这些数据表明，凝集素可以识别出似乎与Ad2转化细胞系中的恶性潜能相关的表面特性。
