Griffin J D, Hercend T, Beveridge R, Schlossman S F
J Immunol. 1983 Jun;130(6):2947-51.
A monoclonal antibody, anti-N901, was produced by fusing NS-1 myeloma cells with spleen cells of a mouse immunized with human CML cells. This antibody was reactive with a subpopulation of peripheral blood LGL, including the natural killer cells. Monocytes, granulocytes, B cells, T cells (T3+ cells), erythrocytes, and platelets were nonreactive. The N901-positive cells in the peripheral blood were heterogeneous with respect to expression of other cell surface antigens. The majority of N901+ cells co-expressed T11, Mo1, and HNK-1, whereas a smaller percentage expressed T8. Ia, T3, T4, Mo2, or B1 antigens were very uncommon on N901+ cells. The heterogeneity of the N901+ LGL was further investigated by examining the expression of N901 antigen on a series of cloned normal human NK cell lines. N901 antigen was expressed by each of the NK cell lines tested, and by a minority of cloned T cell lines without NK activity. Anti-N901 does not block NK activity and can be used to rapidly purify functional NK cells for further study.
通过将NS-1骨髓瘤细胞与用人慢性粒细胞白血病（CML）细胞免疫的小鼠脾细胞融合，制备了一种单克隆抗体——抗-N901。该抗体与外周血大颗粒淋巴细胞（LGL）的一个亚群发生反应，包括自然杀伤细胞。单核细胞、粒细胞、B细胞、T细胞（T3+细胞）、红细胞和血小板无反应。外周血中N901阳性细胞在其他细胞表面抗原的表达方面具有异质性。大多数N901+细胞共表达T11、Mo1和HNK-1，而较小比例的细胞表达T8。Ia、T3、T4、Mo2或B1抗原在N901+细胞上非常罕见。通过检测一系列克隆的正常人自然杀伤细胞系上N901抗原的表达，进一步研究了N901+ LGL的异质性。所测试的每个自然杀伤细胞系以及少数无自然杀伤活性的克隆T细胞系均表达N901抗原。抗-N901不阻断自然杀伤活性，可用于快速纯化功能性自然杀伤细胞以进行进一步研究。
