Abo T, Cooper M D, Balch C M
J Immunol. 1982 Oct;129(4):1752-7.
Human lymphocytes with NK and K cell activities can be identified by the HNK-1 (Leu-7) monoclonal antibody. In these experiments, subsets of HNK-1+ cells from blood and bone marrow were distinguished by their expression of other cell surface antigens, their morphology, and their NK functional capability. Two-color immunofluorescence analysis revealed that subpopulations of HNK-1+ cells in blood expressed antigens found on mature T cells (e.g., T1, T3, T4, T8), but none expressed antigens characteristic of immature T cells (T6, T9). The majority of HNK-1+ cells (greater than 60%) also expressed a myeloid antigen (M1), whereas a minority (less than 25%) expressed HLA-DR. HNK-1+ cells were separated into T3- and T3+ subsets with the fluorescence-activated cell sorter and analyzed for their morphology and NK cell function. HNK+T3- cells exhibited a high level of NK activity against K562 target cells and contained many cytoplasmic granules. On the other hand, HNK+T3+ cells had low NK activity and a paucity of cytoplasmic granules. The cell sizes of HNK+T3- and HNK+T3+ cells were indistinguishable by light-scatter analysis. When these cell fractions were analyzed further, a reciprocal relationship between T3 and M1 antigen expression was observed. These results thus delineate two distinct subsets of human HNK-1+ cells in blood with different cytotoxic capability: HNK+T3-M1+ and HNK+T3+M1- cells. Analysis of bone marrow cells demonstrated that only 0.7% of the nucleated cells expressed the HNK-1 antigen; virtually all of these cells expressed both the T3 and T8 antigens but lacked the M1 antigen. Thus, a majority of HNK-1+ cell population in blood were T3-M1+, whereas almost all bone marrow HNK-1+ cells were T3+M1-. We propose that these subsets of cells represent different stages in NK cells differentiation.
具有自然杀伤（NK）和杀伤（K）细胞活性的人淋巴细胞可用HNK-1（Leu-7）单克隆抗体识别。在这些实验中，通过其他细胞表面抗原的表达、形态学以及NK功能能力，区分了来自血液和骨髓的HNK-1+细胞亚群。双色免疫荧光分析显示，血液中HNK-1+细胞亚群表达成熟T细胞上发现的抗原（如T1、T3、T4、T8），但没有一个表达未成熟T细胞的特征性抗原（T6、T9）。大多数HNK-1+细胞（超过60%）也表达一种髓系抗原（M1），而少数（少于25%）表达HLA-DR。用荧光激活细胞分选仪将HNK-1+细胞分为T3-和T3+亚群，并分析其形态和NK细胞功能。HNK+T3-细胞对K562靶细胞表现出高水平的NK活性，并含有许多细胞质颗粒。另一方面，HNK+T3+细胞的NK活性低，细胞质颗粒少。通过光散射分析，HNK+T3-和HNK+T3+细胞的细胞大小无法区分。当对这些细胞组分进行进一步分析时，观察到T3和M1抗原表达之间的相互关系。因此，这些结果描绘了血液中具有不同细胞毒性能力的两种不同的人HNK-1+细胞亚群：HNK+T3-M1+和HNK+T3+M1-细胞。骨髓细胞分析表明，只有0.7%的有核细胞表达HNK-1抗原；实际上所有这些细胞都表达T3和T8抗原，但缺乏M1抗原。因此，血液中大多数HNK-1+细胞群体是T3-M1+，而几乎所有骨髓HNK-1+细胞是T3+M1-。我们认为这些细胞亚群代表了NK细胞分化的不同阶段。
