Carbonell F, Hoelzer D, Grilli G, Issaragrisil S, Harriss E B, Fliedner T M
Scand J Haematol. 1983 May;30(5):486-91. doi: 10.1111/j.1600-0609.1983.tb02537.x.
In 20 patients with chronic myelocytic leukaemia in the chronic phase or in blast crisis, several in vitro (CFU-C, BFU-E) and in vivo (diffusion chamber) culture techniques were used to demonstrate the existence of a still diploid cell clone. In 4 out of 12 patients in the chronic phase, Ph1-negative metaphases were found in the various cultures for haemopoietic progenitor cells but not in the standard suspension cultures used for cytogenetics. However, all the 8 patients investigated during blast crisis had only Ph1-positive and other abnormal clones, indicating the loss of the diploid clone during progression of the disease.
在20例处于慢性期或急变期的慢性粒细胞白血病患者中，采用了几种体外（CFU-C、BFU-E）和体内（扩散室）培养技术来证明仍为二倍体细胞克隆的存在。在12例慢性期患者中的4例中，在各种造血祖细胞培养物中发现了Ph1阴性中期细胞，但在用于细胞遗传学的标准悬浮培养物中未发现。然而，在急变期研究的所有8例患者中，只有Ph1阳性和其他异常克隆，表明在疾病进展过程中二倍体克隆消失。
