Greenberg B R, Wilson F D, Woo L, Jenks H M
Blood. 1978 Jun;51(6):1039-44.
The cytogenetic status of bone marrow stromal elements obtained from six patients with Ph1-positive chronic myelogenous leukemia (CML), two in blast crisis, was studied in vitro utilizing the potential of marrow to form surface-adherent colonies morphologically compatible with mesenchymal elements. We demonstrated the absence of both the marker chromosome and other chromosomal abnormalities in all the fibroblastic colonies studied, indicating that the progenitors of such colonies (plaque-forming units in culture, PFU-C) are not closely related to hematopoietic elements including macrophages. This supports previous reports suggesting that the stromal elements in myelofibrosis associated with CML are not derived from the primary Ph1-positive malignant clone but represent a stromal reactive component of benign or independent malignant potential.
对6例Ph1阳性慢性粒细胞白血病（CML）患者（其中2例处于急变期）的骨髓基质成分的细胞遗传学状态进行了体外研究，利用骨髓形成形态与间充质成分相符的表面贴壁集落的能力。我们发现，在所研究的所有成纤维细胞集落中，标记染色体和其他染色体异常均不存在，这表明此类集落的祖细胞（培养中的集落形成单位，PFU-C）与包括巨噬细胞在内的造血成分没有密切关系。这支持了先前的报道，即与CML相关的骨髓纤维化中的基质成分并非源自原发性Ph1阳性恶性克隆，而是代表具有良性或独立恶性潜能的基质反应性成分。
