变形链球菌诱导的BALB/c小鼠龋齿的可重复性

Reproducibility of dental caries in BALB/c mice induced by the bacterium Streptococcus mutans.

作者信息

Kamp E M, Drost J, Huis in 't Veld J H, van Palenstein Helderman W H, Dirks O B

出版信息

Arch Oral Biol. 1983;28(2):153-8. doi: 10.1016/0003-9969(83)90122-x.

Abstract

Dental caries induced by infection with Strep. mutans C67-1 (serotype c) or Strep. mutans 50B4 (serotype d/g) and feeding a 30 per cent sucrose-containing diet was found mainly in fissures; smooth surface caries was not detected. A decrease in the mean caries score was observed with time, coinciding with lower recoveries of Strep. mutans at the end of the experimental period. The decrease in caries formation may be attributed to changes in some of the cariogenic properties of the individual Strep. mutans strains during maintenance in the laboratory, as re-isolation of the strains from caries-active mice yielded similar and reproducible caries scores to those in early experiments. Hence Balb/c mice can be used as an experimental model in caries research, provided that great care is taken in selecting and storing the bacteria.

摘要

感染变形链球菌C67 - 1（血清型c）或变形链球菌50B4（血清型d/g）并喂食含30%蔗糖饮食诱导产生的龋齿主要出现在裂沟处；未检测到平滑面龋。随着时间推移，平均龋损评分下降，这与实验期末变形链球菌回收率降低相一致。龋损形成的减少可能归因于实验室保存期间个别变形链球菌菌株的一些致龋特性发生了变化，因为从患龋活性小鼠中重新分离出的菌株产生的龋损评分与早期实验相似且可重复。因此，只要在选择和保存细菌时格外小心，Balb/c小鼠可作为龋齿研究的实验模型。