Obtention of rat monoclonal antibodies reactive with human leukaemic lymphoblasts.

The Louvain rat-rat hybridoma technology was used to analyse membrane antigens present on human leukaemic lymphoblasts. Three fusions directed against non T non B leukaemic lymphoblasts finally yielded 42 distinct, stable, and specific antibody-secreting hybridomas. The cellular and molecular specificities of these monoclonal antibodies are still under investigation. However, a striking feature of our preliminary characterization is the obtention, in single fusions, of several distinct hybridomas that secrete antibodies binding to the same membrane molecule.