Heard J M, Fichelson S, Sola B, Martial M A, Berger R, Varet B, Levy J P
Int J Cancer. 1983 Aug 15;32(2):237-45. doi: 10.1002/ijc.2910320216.
A Friend helper virus I-5(F-MuLV) which induces myeloblastic leukemias in mice after a latency of several months, was used to infect long-term bone marrow cultures. From 48 to 71 weeks after in vitro infection, 4/14 cultures gave rise to transplantable malignant myeloblastic cells. These cells were shown to genuinely result from as in vitro transformation of virus-infected normal bone marrow cells. The in vitro transformation reproduced the course of the in vivo disease. It provided unique material for in vitro investigation of the preleukemic stages of long-term leukemogenesis. Successive cellular events were: (1) freezing of the normal myelomonocytic differentiation process; (2) change from factor-dependent to an autonomous growth; (3) acquisition of in vivo tumorigenicity.
一种Friend辅助病毒I-5（F-MuLV），在潜伏数月后可诱发小鼠的髓母细胞性白血病，被用于感染长期骨髓培养物。体外感染后48至71周，14个培养物中有4个产生了可移植的恶性髓母细胞。这些细胞被证明确实是病毒感染的正常骨髓细胞体外转化的结果。体外转化重现了体内疾病的病程。它为长期白血病发生的白血病前期阶段的体外研究提供了独特的材料。连续的细胞事件为：（1）正常髓单核细胞分化过程的停滞；（2）从因子依赖型生长转变为自主生长；（3）获得体内致瘤性。
