Clements P R, Mahuran D J, Hopwood J J
J Chromatogr. 1983 May 20;261(1):77-82. doi: 10.1016/s0021-9673(01)87920-6.
Elution of bound glycoproteins from concanavalin A-Sepharose can be made more efficient by their readsorption to a Blue A agarose column (specific) and Green A agarose column (less-specific) during recycling of the elution buffer. Three lysosomal enzymes were eluted in this way with marked improvement in their specific activities, time and handling and amount of eluting ligand used.
在洗脱缓冲液循环过程中，通过将结合的糖蛋白再吸附到蓝色琼脂糖柱（特异性）和绿色琼脂糖柱（非特异性较低）上，可以提高伴刀豆球蛋白A-琼脂糖上结合糖蛋白的洗脱效率。以这种方式洗脱了三种溶酶体酶，其比活性、时间、操作以及洗脱配体的用量都有显著改善。
