布氏锥虫在体外合成变异特异性抗原。
The synthesis of a variant-specific antigen by Trypanosoma brucei in vitro.
作者信息
Taylor D W, Cross G A
出版信息
Parasitology. 1977 Feb;74(1):47-60. doi: 10.1017/s0031182000047521.
A variant-specific surface antigen from a cloned population of Trypanosoma brucei S42 has been isolated and partially characterized. [35S]L-methionine was found to be incorporated into this material by cells incubated in vitro in a chemically defined medium. Incorporation of [35S]L-methionine was inhibited by cycloheximide and puromycin at concentrations which are known to specifically inhibit protein synthesis in other systems. The rate of synthesis of the variant-specific antigen in vitro has been estimated to be about 8% of the rate in vivo. Newly synthesized [35S]L-methionine-labelled variant-specific antigen was incorporated into the surface coat.
已从布氏锥虫S42的克隆群体中分离出一种变体特异性表面抗原，并对其进行了部分特性鉴定。发现[35S]L-甲硫氨酸可被体外培养于化学成分明确的培养基中的细胞掺入该物质中。环己酰亚胺和嘌呤霉素在已知能特异性抑制其他系统中蛋白质合成的浓度下可抑制[35S]L-甲硫氨酸的掺入。体外变体特异性抗原的合成速率估计约为体内合成速率的8%。新合成的[35S]L-甲硫氨酸标记的变体特异性抗原被掺入表面被膜中。
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