Moriuchi T, Oikawa T, Kodama T, Yamaguchi H, Kobayashi H
Cancer Res. 1983 Nov;43(11):5478-83.
A transplantable myelomonocytic leukemia was established from a leukemia of a WKA/Hok rat which had been inoculated with Rauscher virus at birth. The tumor grew in ascites form in normal syngeneic rats and, after the middle stage of i.p. transplantation, leukemia cells consisting of a mixed population of monocytic and granulocytic cells were observed in the peripheral blood. A complement-dependent cytotoxicity test failed to demonstrate Rauscher virus-related antigen on the tumor cell surface. Membrane marker analysis revealed that most of the tumor cells possessed receptors for both complement and neuraminidase-treated sheep RBC. More than 90% of ascitic tumor cells displayed phagocytic activity and a positive nonspecific esterase reaction. Serum from rats bearing this tumor contained high levels of muramidase. Ultrastructurally, the tumor cells resembled both immature and mature cells of the monocyte-macrophage series. On serial transplantation into the peritoneal cavity, the tumor displayed consistent differentiation from undifferentiated blast cells to monocytes and cells indistinguishable from granulocytes. The karyotype analysis revealed that the modal number of chromosomes of the tumor cells was 81, and no structural abnormalities of chromosomes were observed after quinacrine mustard staining. This transplantable leukemia will provide a useful experimental model for the study of granulocyte-monocyte differentiation and for human myelomonocytic leukemia.
一种可移植的骨髓单核细胞白血病是从一只出生时接种劳斯氏病毒的WKA/Hok大鼠的白血病中建立的。该肿瘤在同基因正常大鼠中以腹水形式生长，在腹腔内移植中期后，在外周血中观察到由单核细胞和粒细胞混合群体组成的白血病细胞。补体依赖性细胞毒性试验未能证明肿瘤细胞表面存在与劳斯氏病毒相关的抗原。膜标记分析显示，大多数肿瘤细胞同时具有补体和神经氨酸酶处理的绵羊红细胞的受体。超过90%的腹水肿瘤细胞表现出吞噬活性和阳性非特异性酯酶反应。携带这种肿瘤的大鼠血清中含有高水平的溶菌酶。在超微结构上，肿瘤细胞类似于单核细胞-巨噬细胞系列的未成熟和成熟细胞。在连续移植到腹腔后，肿瘤显示出从未分化的原始细胞到单核细胞以及与粒细胞难以区分的细胞的一致分化。核型分析显示，肿瘤细胞的染色体众数为81，在用喹吖因芥子染色后未观察到染色体结构异常。这种可移植白血病将为研究粒细胞-单核细胞分化以及人类骨髓单核细胞白血病提供一个有用的实验模型。
