佛波酯相关的神经节苷脂代谢变化。
Phorbol ester-associated changes in ganglioside metabolism.
作者信息
Burczak J D, Moskal J R, Trosko J E, Fairley J L, Sweeley C C
出版信息
Exp Cell Res. 1983 Sep;147(2):281-6. doi: 10.1016/0014-4827(83)90210-0.
The effect of phorbol esters on ganglioside metabolism in contact-inhibited Chinese hamster V79 cells was examined. Three phorbol esters of varying structure and tumor-promoting activity were used. Treatment of cells with tumor-promoting phorbol esters resulted in accumulation of gangliosides and increased incorporation of [1-14C]palmitate and [9-3H]sialic acid into gangliosides. Moreover, the phorbol esters were found to increase the activity of CMP-sialic acid: lactosylceramide sialyltransferase, the enzyme catalysing the first step in ganglioside biosynthesis. The magnitude of phorbol ester effects on V79 cell ganglioside metabolism correlated with the in vivo phorbol ester tumor-promoting activity.
研究了佛波酯对接触抑制的中国仓鼠V79细胞中神经节苷脂代谢的影响。使用了三种结构和肿瘤促进活性不同的佛波酯。用具有肿瘤促进作用的佛波酯处理细胞会导致神经节苷脂积累，并增加[1-14C]棕榈酸酯和[9-3H]唾液酸掺入神经节苷脂的量。此外，发现佛波酯可增加CMP-唾液酸：乳糖基神经酰胺唾液酸转移酶的活性，该酶催化神经节苷脂生物合成的第一步。佛波酯对V79细胞神经节苷脂代谢的影响程度与体内佛波酯的肿瘤促进活性相关。
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