Enomoto T, Martel N, Kanno Y, Yamasaki H
J Cell Physiol. 1984 Nov;121(2):323-33. doi: 10.1002/jcp.1041210209.
The effect of phorbol ester tumor promoters on the communication between individual cells in confluent culture was studied using a fluorescent dye transfer method. Cell-cell communication between mouse Balb/c 3T3 cells and between Chinese hamster V79 cells was inhibited almost completely by tumor-promoting phorbol esters, but not by nonpromoting derivatives; the effect was reversed upon removal of the promoter. Intercellular communication between Balb/c 3T3 cells, but not Chinese hamster V79 cells, was increased significantly in the presence of dbcAMP and caffeine, and these compounds counteracted the effects of tumor promoters. Inhibition of cell communication by phorbol esters appears to be receptor-mediated, since specific binding of 3H-phorbol-12,13-dibutyrate to Balb/c 3T3 cells was inhibited only by compounds that also inhibit intercellular dye transfer. A study with cycloheximide suggests that the reversible inhibition of intercellular communication by phorbol esters may not need de novo protein synthesis, while upregulation of communication by cAMP requires protein synthesis.
利用荧光染料转移法研究了佛波酯肿瘤促进剂对汇合培养中单个细胞间通讯的影响。促肿瘤佛波酯几乎完全抑制了小鼠Balb/c 3T3细胞之间以及中国仓鼠V79细胞之间的细胞间通讯，但非促肿瘤衍生物则无此作用；去除促进剂后，该作用可逆转。在存在二丁酰环磷腺苷（dbcAMP）和咖啡因的情况下，Balb/c 3T3细胞之间的细胞间通讯显著增加，而中国仓鼠V79细胞则不然，并且这些化合物可抵消肿瘤促进剂的作用。佛波酯对细胞通讯的抑制似乎是受体介导的，因为3H-佛波醇-12,13-二丁酸酯与Balb/c 3T3细胞的特异性结合仅被同样抑制细胞间染料转移的化合物所抑制。用环己酰亚胺进行的一项研究表明，佛波酯对细胞间通讯的可逆抑制可能不需要从头合成蛋白质，而cAMP对通讯的上调则需要蛋白质合成。
