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将非洲爪蟾蝌蚪和成体的β-珠蛋白基因注入非洲爪蟾受精卵后二者的差异表达。

Differential expression of the Xenopus laevis tadpole and adult beta-globin genes when injected into fertilized Xenopus laevis eggs.

作者信息

Bendig M M, Williams J G

出版信息

Mol Cell Biol. 1984 Mar;4(3):567-70. doi: 10.1128/mcb.4.3.567-570.1984.

DOI:10.1128/mcb.4.3.567-570.1984
PMID:6717434
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC368738/
Abstract

Xenopus laevis tadpole and adult beta-globin genes were injected into fertilized X. laevis eggs. Both injected genes replicated and were retained in the developing embryos with equal efficiency. Transcripts of the injected adult gene were detectable at gastrulation and reached a maximum level shortly thereafter. In contrast, transcripts of the injected tadpole gene were not detected until much later stages of development. The level of expression of both the injected genes was low compared with the level of expression of the chromosomal genes during erythropoiesis.

摘要

将非洲爪蟾蝌蚪和成体的β-珠蛋白基因注射到非洲爪蟾的受精卵中。两种注射的基因都能复制，并以相同效率保留在发育中的胚胎中。在原肠胚形成期可检测到注射的成体基因的转录本，此后不久达到最高水平。相比之下，注射的蝌蚪基因的转录本直到发育后期才被检测到。与红细胞生成过程中染色体基因的表达水平相比，两种注射基因的表达水平都很低。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/9b92/368738/391c2eabb63d/molcellb00145-0189-b.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/9b92/368738/c402f725db9b/molcellb00145-0189-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/9b92/368738/391c2eabb63d/molcellb00145-0189-b.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/9b92/368738/c402f725db9b/molcellb00145-0189-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/9b92/368738/391c2eabb63d/molcellb00145-0189-b.jpg

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Differential expression of the Xenopus laevis tadpole and adult beta-globin genes when injected into fertilized Xenopus laevis eggs.将非洲爪蟾蝌蚪和成体的β-珠蛋白基因注入非洲爪蟾受精卵后二者的差异表达。
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