• 文献检索
  • 文档翻译
  • 深度研究
  • 学术资讯
  • Suppr Zotero 插件Zotero 插件
  • 邀请有礼
  • 套餐&价格
  • 历史记录
应用&插件
Suppr Zotero 插件Zotero 插件浏览器插件Mac 客户端Windows 客户端微信小程序
定价
高级版会员购买积分包购买API积分包
服务
文献检索文档翻译深度研究API 文档MCP 服务
关于我们
关于 Suppr公司介绍联系我们用户协议隐私条款
关注我们

Suppr 超能文献

核心技术专利：CN118964589B侵权必究
粤ICP备2023148730 号-1Suppr @ 2026

文献检索

告别复杂PubMed语法，用中文像聊天一样搜索，搜遍4000万医学文献。AI智能推荐，让科研检索更轻松。

立即免费搜索

文件翻译

保留排版，准确专业，支持PDF/Word/PPT等文件格式，支持 12+语言互译。

免费翻译文档

深度研究

AI帮你快速写综述，25分钟生成高质量综述，智能提取关键信息，辅助科研写作。

立即免费体验

甲氨蝶呤浓度和暴露时间对体外培养的哺乳动物细胞存活的影响。

Effect of methotrexate concentration and exposure time on mammalian cell survival in vitro.

作者信息

Eichholtz H, Trott K R

出版信息

Br J Cancer. 1980 Feb;41(2):277-84. doi: 10.1038/bjc.1980.40.

DOI:10.1038/bjc.1980.40
PMID:7370167
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC2010205/
Abstract

Chinese hamster, HeLa and HAK cells were treated with methotrexate (MTX) to determine the dependence of its effect on drug concentration and exposure time. With a broad range of survival curves for Chinese hamster cells, cell survival is an exponential function of exposure time and a power function of drug concentration. The data allow a mathematical description to be made of the interdependence of MTX concentration and drug exposure in relation to cell survival.

摘要

用甲氨蝶呤（MTX）处理中国仓鼠、HeLa和HAK细胞，以确定其作用对药物浓度和暴露时间的依赖性。中国仓鼠细胞具有广泛的存活曲线，细胞存活是暴露时间的指数函数和药物浓度的幂函数。这些数据允许对甲氨蝶呤浓度与药物暴露在细胞存活方面的相互依赖性进行数学描述。

相似文献

1
Effect of methotrexate concentration and exposure time on mammalian cell survival in vitro.甲氨蝶呤浓度和暴露时间对体外培养的哺乳动物细胞存活的影响。
Br J Cancer. 1980 Feb;41(2):277-84. doi: 10.1038/bjc.1980.40.
2
Dependence of the cytostatic effect of adriamycin on drug concenration and exposure time in vitro.阿霉素体外细胞抑制作用对药物浓度和暴露时间的依赖性。
Br J Cancer. 1980 Jun;41(6):886-91. doi: 10.1038/bjc.1980.165.
3
Inhibition of DNA excision repair by methotrexate in Chinese hamster ovary cells following exposure to ultraviolet irradiation or ethylmethanesulfonate.甲氨蝶呤对紫外线照射或甲基磺酸乙酯处理后的中国仓鼠卵巢细胞DNA切除修复的抑制作用。
Cancer Res. 1990 Mar 15;50(6):1786-9.
4
Methotrexate cytotoxicity for L5178Y/Asn- lymphoblasts: relationship of dose and duration of exposure to tumor cell viability.甲氨蝶呤对L5178Y/Asn-淋巴母细胞的细胞毒性：暴露剂量和持续时间与肿瘤细胞活力的关系。
Cancer Res. 1982 May;42(5):1641-5.
5
Reversal of resistance to methotrexate by hyperthermia in Chinese hamster ovary cells.热疗对中国仓鼠卵巢细胞中甲氨蝶呤耐药性的逆转作用。
Cancer Res. 1981 Oct;41(10):3840-3.
6
The relationship between cisplatin sensitivity and drug uptake into mammalian cells in vitro.顺铂敏感性与体外药物摄入哺乳动物细胞之间的关系。
Br J Cancer. 1986 Aug;54(2):239-43. doi: 10.1038/bjc.1986.168.
7
Effect of tumor promoter 12-O-tetradecanoylphorbol-13-acetate on recovery of methotrexate-, N-(phosphonacetyl)-L-aspartate-, and cadmium-resistant colony-forming mouse and hamster cells.肿瘤启动子12-O-十四酰佛波醇-13-乙酸酯对甲氨蝶呤、N-（膦酰乙酰基）-L-天冬氨酸和镉抗性集落形成小鼠及仓鼠细胞恢复的影响。
Cancer Res. 1983 Nov;43(11):5217-21.
8
[Effect of methotrexate (MTX) on neoplastic (HeLa line) and normal (lymphocyte) cells].
Rev Esp Oncol. 1982;29(4):649-64.
9
Resistance of resting 3T6 mouse fibroblasts to methotrexate cytotoxicity.
Cancer Res. 1978 Aug;38(8):2408-12.
10
Collateral sensitivity to methotrexate in cells resistant to adriamycin.对阿霉素耐药的细胞对甲氨蝶呤的 collateral 敏感性 。（注：“collateral”在这里可能有特定专业含义，比如“旁系的、间接的”等，具体需结合医学专业知识准确理解其确切意义）
Cancer Res. 1979 Jun;39(6 Pt 1):1937-42.

引用本文的文献

1
A slow-release methotrexate formulation for intrathecal chemotherapy.
Cancer Chemother Pharmacol. 1993;32(3):179-82. doi: 10.1007/BF00685832.
2
An extended-release formulation of methotrexate for subcutaneous administration.一种用于皮下给药的甲氨蝶呤缓释制剂。
Cancer Chemother Pharmacol. 1994;33(4):303-6. doi: 10.1007/BF00685904.
3
Dependence of the cytostatic effect of adriamycin on drug concenration and exposure time in vitro.阿霉素体外细胞抑制作用对药物浓度和暴露时间的依赖性。
Br J Cancer. 1980 Jun;41(6):886-91. doi: 10.1038/bjc.1980.165.
4
Long-duration intracavitary infusion of methotrexate with systemic leucovorin protection in patients with malignant effusions.甲氨蝶呤长期腔内输注联合全身亚叶酸钙保护用于恶性积液患者的治疗
J Clin Invest. 1981 Apr;67(4):1161-70. doi: 10.1172/jci110130.
5
Quantitation of chemosensitivity in acute myelocytic leukaemia.急性髓细胞白血病化疗敏感性的定量分析
Br J Cancer. 1983 Oct;48(4):559-67. doi: 10.1038/bjc.1983.229.
6
Comparison of serum and cerebrospinal fluid levels of methotrexate in man during high-dose chemotherapy for aggressive non-Hodgkin's lymphoma.侵袭性非霍奇金淋巴瘤大剂量化疗期间人体血清和脑脊液中甲氨蝶呤水平的比较。
Cancer Chemother Pharmacol. 1985;15(3):290-4. doi: 10.1007/BF00263903.

本文引用的文献

1
Hydroxyurea: differential lethal effects on cultured mammalian cells during the cell cycle.羟基脲：在细胞周期中对培养的哺乳动物细胞的差异性致死效应。
Science. 1965 Dec 24;150(3704):1729-31. doi: 10.1126/science.150.3704.1729.
2
Comparison of the dose- and timesurvival curves for normal hematopoietic and lymphoma colony-forming cells exposed to vinblastine, vincristine, arabinosylcytosine, and amethopterin.暴露于长春碱、长春新碱、阿糖胞苷和氨甲蝶呤的正常造血和淋巴瘤集落形成细胞的剂量-时间存活曲线比较。
J Natl Cancer Inst. 1969 Jun;42(6):1015-25.
3
Cell killing studies on the mode of action of methotrexate on L-cells in vitro.
Cancer Res. 1969 Apr;29(4):737-44.
4
Some observations on the combined effects of x-rays and methotrexate on human tumor cells in vitro with possible relevance to their most useful combination in radiotherapy.
Am J Roentgenol Radium Ther Nucl Med. 1968 Mar;102(3):509-18. doi: 10.2214/ajr.102.3.509.
5
Carrier-mediated transport of the folic acid analogue, methotrexate, in the L1210 leukemia cell.叶酸类似物甲氨蝶呤在L1210白血病细胞中的载体介导转运
J Biol Chem. 1968 Oct 10;243(19):5007-17.
6
On the nature of permeability of sarcoma-180 cells to amethopterin in vitro.肉瘤-180细胞在体外对氨甲蝶呤的通透性性质
Biochim Biophys Acta. 1965 May 25;102(1):210-25. doi: 10.1016/0926-6585(65)90214-1.
7
Progress report on high-dose methotrexate (NSC-740) with citrovorum rescue in the treatment of metastatic bone tumors.大剂量甲氨蝶呤（NSC-740）联合亚叶酸钙解救治疗转移性骨肿瘤的进展报告
Cancer Chemother Rep. 1974 Mar-Apr;58(2):275-80.
8
Phase I study of high doses of methotrexate with citrovorum factor in patients with lung cancer.
Cancer. 1972 Jul;30(1):22-30. doi: 10.1002/1097-0142(197207)30:1<22::aid-cncr2820300105>3.0.co;2-b.
9
Active transport of aminopterin in Yoshida sarcoma cells.
Biochim Biophys Acta. 1967;135(5):927-36. doi: 10.1016/0005-2736(67)90062-4.
10
An effective low-dose intermittent cyclophosphamide, methotrexate, and 5-fluorouracil treatment regimen for metastatic breast cancer.
Cancer. 1975 Apr;35(4):1101-7. doi: 10.1002/1097-0142(197504)35:4<1101::aid-cncr2820350413>3.0.co;2-m.