Spirande I V
Biull Eksp Biol Med. 1983 Oct;96(10):83-4.
A mutant (HPRT-) cell line was obtained by a series of passages in the medium with 8-azaguanine on the basis of human erythromyeloleukemia K-562 cells. Rapid total death of the cells in a selective medium containing hypoxanthine, aminopterine and thymidine (3 to 4 passages) supported the deficiency as regards hypoxanthine phosphoribosyltransferase. Preliminary experiments aimed at cytotoxicity studies demonstrated that the new mutant cell line lost the property, common to the parent K-562 line, of being sensitive to the attack of natural killers.
在人红白血病K-562细胞的基础上，通过在含有8-氮杂鸟嘌呤的培养基中进行一系列传代培养，获得了一种突变（HPRT-）细胞系。在含有次黄嘌呤、氨基蝶呤和胸腺嘧啶核苷的选择性培养基中细胞迅速全部死亡（传代3至4次），这证实了次黄嘌呤磷酸核糖基转移酶缺乏。旨在进行细胞毒性研究的初步实验表明，新的突变细胞系失去了亲代K-562细胞系所共有的对自然杀伤细胞攻击敏感的特性。
