Andreu D, Merrifield R B, Steiner H, Boman H G
Proc Natl Acad Sci U S A. 1983 Nov;80(21):6475-9. doi: 10.1073/pnas.80.21.6475.
Cecropin A, a 37-residue antibacterial peptide amide, was synthesized by the solid-phase method. It was shown to be homogeneous and totally indistinguishable from natural cecropin A by chemical and physical criteria, as well as by its antibacterial activity against several Gram-positive and Gram-negative organisms. The synthetic material was also used to establish unambiguously that the carboxyl-terminal blocking group of natural cecropin A is a primary amide as tentatively proposed earlier. The role of the amino terminus of cecropin A in antibacterial activity was investigated by the synthesis of two analogs.
天蚕素A是一种由37个氨基酸残基组成的抗菌肽酰胺，采用固相法合成。通过化学、物理标准以及对几种革兰氏阳性菌和革兰氏阴性菌的抗菌活性表明，合成的天蚕素A是均一的，与天然天蚕素A完全没有区别。合成材料还被用于明确证实天然天蚕素A的羧基末端封闭基团是一个伯酰胺，这与之前初步推测的一致。通过合成两种类似物研究了天蚕素A氨基末端在抗菌活性中的作用。
