Schlaff S, Kobayashi Y, Wright K H, Santulli R, Wallach E E
Prostaglandins. 1983 Jul;26(1):111-21. doi: 10.1016/0090-6980(83)90079-5.
PGF2a has been proposed as a mediator of mammalian ovulation. To elucidate further the role of PGF2a in the process of ovulation, PGF and PGF2a metabolite were measured by radioimmunoassay in the perfusate of an in vitro perfused rabbit ovary preparation. Perfusion medium samples were collected over a 10 to 12 hour period from ovaries perfused with tissue culture M199 (total volume 150 ml, sample volume 3 ml) to which varying amounts of hCG had been added. [The PGF2a antisera a 40% cross reaction with PGF1a, hence total PGF was measured with this antisera.] Both PGF and PGF2a metabolite showed a linear increase with time and numbers of ovulations. PGF media accumulation was 575 pg/ovary/ovulation/hr and PGF2a metabolite accumulation was 367 pg/ovary/ovulation/hr. Medium prostaglandin content could be correlated with numbers of ovulations, ovulatory efficiency (number of ovulations/total follicles) but not total follicles. These data best fit a model of independent ovulatory units producing PGF2a without recruitment or interaction between them. We infer that PGF and PGF2a metabolites in this system can be used as a direct index of the ovulation process.
前列腺素F2α（PGF2α）被认为是哺乳动物排卵的介质。为了进一步阐明PGF2α在排卵过程中的作用，采用放射免疫分析法测定了体外灌注兔卵巢制剂灌流液中的PGF和PGF2α代谢物。在10至12小时内，从用添加了不同量人绒毛膜促性腺激素（hCG）的组织培养液M199灌注（总体积150毫升，样本体积3毫升）的卵巢中收集灌注培养基样本。[PGF2α抗血清与PGF1α有40%的交叉反应，因此用该抗血清测定总PGF。]PGF和PGF2α代谢物均随时间和排卵数呈线性增加。PGF在培养基中的积累量为575皮克/卵巢/排卵/小时，PGF2α代谢物的积累量为367皮克/卵巢/排卵/小时。培养基中前列腺素含量与排卵数、排卵效率（排卵数/总卵泡数）相关，但与总卵泡数无关。这些数据最符合一个独立排卵单位产生PGF2α而它们之间无募集或相互作用的模型。我们推断，该系统中的PGF和PGF2α代谢物可作为排卵过程的直接指标。
