Chaudhry I M, Kumar R, Waterhouse J P, Chambers D A
Arch Oral Biol. 1983;28(8):741-4. doi: 10.1016/0003-9969(83)90110-3.
Alkaline phosphatase, confined to myoepithelial cells and blood capillaries in rat submandibular salivary gland (SSG), may participate in the regulation of salivary flow. To determine whether the alkaline phosphatase of SSG has unique properties, comparative kinetic and inhibition studies on enzymes from SSG, intestine and kidney were performed. The Km values (at optimal pH for each tissue) of 0.34, 0.55 and 0.49 mM with p-nitrophenylphosphate for the enzymes from SSG, kidney and small intestine respectively were similar. However, in the presence of cadmium the Ki values of 0.08 and 0.12 microM for the enzymes from SSG and kidney respectively were different from the value of 1.86 microM for the enzyme from small intestine. Differences in Ki values suggest differences in biochemical properties between the enzyme from small intestine and that isolated from SSG or kidney.
碱性磷酸酶局限于大鼠下颌下唾液腺（SSG）的肌上皮细胞和毛细血管中，可能参与唾液分泌的调节。为了确定SSG的碱性磷酸酶是否具有独特性质，对来自SSG、肠道和肾脏的酶进行了比较动力学和抑制研究。SSG、肾脏和小肠的酶分别以对硝基苯磷酸酯为底物时，其Km值（在各组织的最适pH下）分别为0.34、0.55和0.49 mM，较为相似。然而，在镉存在的情况下，SSG和肾脏的酶的Ki值分别为0.08和0.12 microM，与小肠的酶的1.86 microM不同。Ki值的差异表明小肠的酶与从SSG或肾脏分离出的酶在生化性质上存在差异。
