Kanazawa Y, Kuramata T, Matsumoto K
Jpn J Antibiot. 1983 Aug;36(8):2065-72.
Susceptibilities of 198 strains of 31 bacterial species to cefmetazole (CMZ) were determined by the 2-fold agar dilution method in parallel with the diameter of inhibition zone by the single-disc method, under the experimental condition established by Kanzazwa. The experiments demonstrated significant correlation between MIC by the dilution method and diameter of inhibition zone in each of conventional assay of the overnight (about 16 hours) incubation, delayed assay (about 24 hours incubation), and rapid assay (about 3-4 or 5-6 hours incubation), thus confirming applicability of the single-disc assay for CMZ. Analysis of the data obtained by using CMZ disc containing 30 micrograms revealed the primary regression equation to be: D (diameter, mm) = 27.9-11.2 log MIC (micrograms/ml) in conventional assay, D = 34.5-13.8 log MIC (microgram/ml) in delayed assay D = 18.1-6.0 log MIC (microgram/ml) in 3-4 hours rapid assay and D = 22.9-8.5 log MIC (microgram/ml) in 5-6 hours rapid assay, respectively. The range of variations in MICs estimated from the diameter of inhibition zone by the disc test was then calculated in comparison with that in MIC determined by the 2-fold agar dilution assays, as reference for the experimental errors which may be involved in the estimation of MIC of CMZ by the single-disc assay.
采用2倍琼脂稀释法，与单纸片法测定抑菌圈直径并行，在神座建立的实验条件下，测定了31种细菌的198株菌株对头孢美唑（CMZ）的敏感性。实验表明，在常规培养（约16小时）、延迟培养（约24小时）和快速培养（约3 - 4或5 - 6小时）的每种常规试验中，稀释法测定的最低抑菌浓度（MIC）与抑菌圈直径之间存在显著相关性，从而证实了单纸片法对CMZ的适用性。对含30微克CMZ纸片所得数据的分析显示，常规试验中的主要回归方程为：D（直径，mm）= 27.9 - 11.2 log MIC（微克/毫升），延迟试验中D = 34.5 - 13.8 log MIC（微克/毫升），3 - 4小时快速试验中D = 18.1 - 6.0 log MIC（微克/毫升），5 - 6小时快速试验中D = 22.9 - 8.5 log MIC（微克/毫升）。然后，通过纸片试验根据抑菌圈直径估算的MIC变化范围与通过2倍琼脂稀释试验测定的MIC变化范围进行比较，作为单纸片法估算CMZ MIC时可能涉及的实验误差的参考。
