Kanazawa Y, Kuramata T, Matsumoto K
Jpn J Antibiot. 1981 Jul;34(7):1113-9.
Susceptibilities to amikacin of 153 strains of 27 bacterial species were determined by the 2-fold agar dilution method in parallel with the diameter of inhibition zone by the single-disc method. The experiments demonstrated significant correlation between MIC by the dilution method and diameter of inhibition zone in each of conventional assay of the over-night (about 16 hours) incubation, delayed assay (about 24 hours incubation), and rapid assay (after 3-4 or 5-6 hours incubation), thus confirming applicability of the single-disc assay for amikacin. Analysis of the data obtained by using amikacin disc containing 30 micrograms revealed the primary regression equation to be: D (diameter, mm)=23.73-8.55 log MIC (micrograms/ml) in conventional assay, D=29.84-11.53 log MIC (microgram/ml) in delayed assay, D=16.47-4.53 log MIC (microgram/ml) in 3-4 hours rapid assay, and D=19.57-6.19 log MIC (micrograms/ml) in 5-6 hours rapid assay, respectively. The range of variations in MICs estimated from the diameter of inhibition zone by the disc test was then calculated in comparison with that in MIC determined by the two-fold agar dilution assays, as reference for the experimental errors which may be involved in the estimation of MIC of amikacin by the single-disc assay.
采用2倍琼脂稀释法测定了27种细菌的153株菌株对阿米卡星的敏感性，并与单纸片法测定的抑菌圈直径进行了平行比较。实验表明，在过夜（约16小时）培养的常规试验、延迟试验（约24小时培养）和快速试验（3 - 4或5 - 6小时培养后）中，稀释法测定的最低抑菌浓度（MIC）与抑菌圈直径之间均存在显著相关性，从而证实了单纸片法对阿米卡星的适用性。对含30微克阿米卡星纸片所获数据的分析显示，常规试验中的主要回归方程为：D（直径，mm）=23.73 - 8.55 log MIC（微克/毫升），延迟试验中为D = 29.84 - 11.53 log MIC（微克/毫升），3 - 4小时快速试验中为D = 16.47 - 4.53 log MIC（微克/毫升），5 - 6小时快速试验中为D = 19.57 - 6.19 log MIC（微克/毫升）。然后，将通过纸片试验抑菌圈直径估算的MIC变化范围与通过2倍琼脂稀释试验测定的MIC变化范围进行比较，作为单纸片法估算阿米卡星MIC时可能涉及的实验误差的参考。
