Lapidot A, Nissim I, Shaklai M, Samuel R, Liberman U A
Clin Sci (Lond). 1984 Feb;66(2):147-53. doi: 10.1042/cs0660147.
Turnover parameters for plasma glycine were measured by administration of a single dose of [15N]glycine to overnight fasted healthy volunteers, nine patients with chronic leukaemia, one patient with acute monocytic leukaemia and one patient with chronic myeloid leukaemia before and after chemotherapy. Gas chromatography-mass spectrometry was used to determine plasma [15N]glycine enrichment. Pool sizes and turnover rate constants were estimated from time-decay curves for isotope enrichment. Turnover rate constants and metabolic clearance rates of plasma glycine in leukaemia patients were elevated in comparison with data for healthy volunteers. The increased rates of glycine disappearance from the circulation were not accompanied by a depletion in glycine pool size. Turnover rate constants fell during chemotherapy and were within the normal range during remission. There was a significant positive correlation between glycine turnover rate constants and leucocyte counts. The increase in glycine turnover rate constants may be related to proliferation of the neoplastic cells.
通过给隔夜禁食的健康志愿者、9例慢性白血病患者、1例急性单核细胞白血病患者和1例慢性粒细胞白血病患者在化疗前后单次服用[15N]甘氨酸来测量血浆甘氨酸的周转参数。采用气相色谱-质谱法测定血浆[15N]甘氨酸富集情况。根据同位素富集的时间衰减曲线估计池大小和周转率常数。与健康志愿者的数据相比，白血病患者血浆甘氨酸的周转率常数和代谢清除率升高。循环中甘氨酸消失率的增加并未伴有甘氨酸池大小的减少。化疗期间周转率常数下降，缓解期内处于正常范围。甘氨酸周转率常数与白细胞计数之间存在显著正相关。甘氨酸周转率常数的增加可能与肿瘤细胞的增殖有关。
