Irving C S, Nissim I, Lapidot A
Biomed Mass Spectrom. 1978 Feb;5(2):117-22. doi: 10.1002/bms.1200050204.
Gas chromatography mass spectrometry has been used to determine the 15N enrichment of plasma glycine of rabbits at various times following the intravenous administration of 15N-glycine. These data were used to prepare isotope enrichment time decay curves for eleven individual animals. The slopes and intercepts of least squares lines that describe the decay curves were considerably more accurately than those reported in similar studies employing radioactive tracers. Individual glycine pool sizes (13.8-37.4 micronmoles per 100 g body wt), turnovers rates (2.66-3.36 pools h-1) and flux (50.4-99.7 micronmoles h-1 per 100 g body wt) were estimated from these parameters in a group of animals and compared with the literature values. These results demonstrate that low risk non-radioactive stable isotopes can be substituted for radioactive tracers in studies of human amino acid metabolism, with considerable saving in time and without loss in accuracy, when gas chromatography mass spectrometry is used to determine plasma amino acid and stable isotope enrichment.
气相色谱 - 质谱联用技术已被用于测定静脉注射15N - 甘氨酸后不同时间兔子血浆甘氨酸的15N富集情况。这些数据被用于为11只个体动物绘制同位素富集时间衰减曲线。描述衰减曲线的最小二乘线的斜率和截距比使用放射性示踪剂的类似研究中报告的结果要准确得多。根据这些参数估算了一组动物的个体甘氨酸池大小（每100克体重13.8 - 37.4微摩尔）、周转率（2.66 - 3.36池/小时）和通量（每100克体重50.4 - 99.7微摩尔/小时），并与文献值进行了比较。这些结果表明，当使用气相色谱 - 质谱联用技术测定血浆氨基酸和稳定同位素富集时，在人类氨基酸代谢研究中，低风险的非放射性稳定同位素可以替代放射性示踪剂，可节省大量时间且不会损失准确性。
