Takigawa M, Okada M, Takano T, Ohmae H, Sakuda M, Suzuki F
J Dent Res. 1984 Jan;63(1):19-22. doi: 10.1177/00220345840630010201.
Methods for isolating chondrocytes from the craniofacial complex and culturing them in vitro were established. Chondrocytes which were isolated by collagenase digestion from mandibular condylar cartilage, nasal septal cartilage, and spheno-occipital synchondrosis grew well in vitro. All three types of chondrocytes actively synthesized glycosaminoglycans, a differentiated phenotype of chondrocytes, and responded well to parathyroid hormone. However, some different characteristics were noted among the three types of chondrocytes in culture.
建立了从颅面复合体分离软骨细胞并进行体外培养的方法。通过胶原酶消化从下颌髁突软骨、鼻中隔软骨和蝶枕软骨联合处分离出的软骨细胞在体外生长良好。所有三种类型的软骨细胞均能积极合成糖胺聚糖，这是软骨细胞的一种分化表型，并且对甲状旁腺激素反应良好。然而，在培养的三种类型软骨细胞中发现了一些不同的特征。
