Poduval T B, Seshadri M, Sundaram K
J Natl Cancer Inst. 1984 Jan;72(1):139-43. doi: 10.1093/jnci/72.1.139.
Combined immunostimulation with BCG and double-stranded polyinosinic-polycytidylic acid (poly I . poly C) was more effective than single-modality immunostimulation in suppressing tumor growth in inbred Swiss mice. BCG sensitization followed by administration of poly I . poly C on the day of tumor cell injection significantly prolonged the survival period against parental lymphosarcoma (LS) and its ascites variant (LS-A). BCG and poly I . poly C given together on the day of tumor cell injection suppressed only LS-A and not LS. BCG or poly I . poly C given alone did not result in tumor cures. Silica injection given 2 days before poly I . poly C injection completely abrogated the antitumor effect of sequential treatment with BCG and poly I . poly C. Silica treatment given on and beyond the day of poly I . poly C injection did not abrogate the antitumor effect. This observation indicated that intact macrophage effector function was essential at the time of tumor cell inoculation to obtain an effective antitumor action.
在近交系瑞士小鼠中，卡介苗（BCG）与双链聚肌苷酸-聚胞苷酸（poly I.poly C）联合免疫刺激在抑制肿瘤生长方面比单一模式免疫刺激更有效。在肿瘤细胞注射当天，先进行卡介苗致敏，然后给予poly I.poly C，可显著延长抗亲本淋巴细胞肉瘤（LS）及其腹水变体（LS-A）的生存期。在肿瘤细胞注射当天同时给予卡介苗和poly I.poly C，仅能抑制LS-A，而不能抑制LS。单独给予卡介苗或poly I.poly C不能治愈肿瘤。在poly I.poly C注射前两天注射二氧化硅，可完全消除卡介苗和poly I.poly C序贯治疗的抗肿瘤作用。在poly I.poly C注射当天及之后进行二氧化硅治疗，不会消除抗肿瘤作用。这一观察结果表明，在接种肿瘤细胞时，完整的巨噬细胞效应功能对于获得有效的抗肿瘤作用至关重要。
